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KMID : 0043319860090030131
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Archives of Pharmacal Research
1986 Volume.9 No. 3 p.131 ~ p.138
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Determination of Barbiturates in Plasma by Gas Chromatography-Flame Photometric Detector after N,N’’-Dimethylthiomethyl Derivatization
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Cho YH
Park MK
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Abstract
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A specific and sensitive gas chromatographic (GC) procedure with the flame photometric detector (FPD) was developed for determination of barbiturates such as barbital, allobarbital, secobarbital, phenobarbital and thiopental in plasma. In order to evaluate the performance of the FPD, the results were campared with those of the flame ionization detector (FID). After extraction of barbiturates from plasma, the barbiturates were quantitatively N,N’’-dimethylthiomethyl (MTM)-derivatized with methylthiomethyl chloride in 1,8-diazabicyclo [5.4.0] undec-7-ene catalyst. The data indicate that the FPD is about 4 times more sensitive than the FID for barbiturates, although it is less reproducible. The FPD also produced chromatogram with less background for extracted plasma sample. The minimum detectable amount of MTM-thiopental on 3% OV-225 column was 4.4fmol and that of other MTM-barbiturate was about 10.0fmol.
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